Plasmid
pNW33N+la

Part:BBa_K3842015:Design

Designed by: Xiaoyi Feng   Group: iGEM21_GA_State_SW_Jiaotong   (2021-10-17)


pNW33N+lac+ EGF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4319
    Illegal EcoRI site found at 6709
    Illegal XbaI site found at 4186
    Illegal PstI site found at 4174
    Illegal PstI site found at 5264
    Illegal PstI site found at 7570
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4319
    Illegal EcoRI site found at 6709
    Illegal NheI site found at 6395
    Illegal NheI site found at 6544
    Illegal PstI site found at 4174
    Illegal PstI site found at 5264
    Illegal PstI site found at 7570
    Illegal NotI site found at 6380
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4319
    Illegal EcoRI site found at 6709
    Illegal BglII site found at 4830
    Illegal BglII site found at 7153
    Illegal BamHI site found at 4192
    Illegal BamHI site found at 7724
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4319
    Illegal EcoRI site found at 6709
    Illegal XbaI site found at 4186
    Illegal PstI site found at 4174
    Illegal PstI site found at 5264
    Illegal PstI site found at 7570
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4319
    Illegal EcoRI site found at 6709
    Illegal XbaI site found at 4186
    Illegal PstI site found at 4174
    Illegal PstI site found at 5264
    Illegal PstI site found at 7570
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 548
    Illegal BsaI.rc site found at 2239
    Illegal BsaI.rc site found at 7644
    Illegal BsaI.rc site found at 9519
    Illegal SapI.rc site found at 1630


Design Notes

PCR mutations


Source

Generated by pNW33N plasmide, T7 promoter, lac operator, EGF coding sequence, T7 terminator.

References